Hi there! thanks for sharing all of this.
So for my case, I am just looking for a system to purge oxygen both from the liquid and head-space before starting the experiment. In order to do so, I work with Luer check valves (unidirectional) from Darwin Microfluidics. (Video Attached), I guess that as I only need to proceed before inoculation… foam formation and potential overflow is something that I should not really worry about.
For me, it would be extremely helpful to automate this directly from the UI (including adding stirring to the purging process), as it would allow me to set up the system inside my chamber and proceed seamlessly. This would also enable more precise control over the purging process by defining specific time intervals and correlating them with temperature and gas dilution rates.
In other setups, I typically work with pressure buildup inside the vial, but I’ve found that gas tends to escape through the tubing, so I instead use a septum-style sealed lid.
I’m really looking forward to seeing the updates that come out of the project you mentioned! Please let me know if there’s anything I can help with.
Thanks!