Hello Everyone,
We are a small group of undergraduate researchers at the University of Toronto working with the PioReactor to grow DNA-transformed E. coli cells. Our immediate goal is to purify the DNA to assess DNA replication. Down the line, we plan to introduce Green Fluorescent Protein plasmids into E. coli cells and cultivating them in the PioReactor, followed by purifying and collecting the proteins. We would appreciate any insights or tips from anyone who has experience with gene expression in the PioReactor, as well as any potential pitfalls to be aware of.