I started my first Pioreactor experiment (control testing) a couple of hours ago , after finally finding the time to prepare some YPD media and sterilizing it in a pressure cooker.
Everything seems to work fine so far, except that I’m getting a “Lost contact with the MQTT server” roughly every two minutes, even though it then almost immediately reconnects? It doesn’t seem to affect any of the readings, but is leading to a pretty noisy event log:
Hm, I’m not sure what’s causing that. Is
worker1 your leader Pioreactor, too? Can you tell me the software version you are using (on the /updates page, titled
No, I’ve got
hmhmhm, I’m not able to reproduce it. A few more questions:
- Is it only the growth-rate-calc job that is reporting this?
- Did it start reporting this almost immediately after starting growth-rate-calc job?
- Did you make any changes to the config.ini, specifically the
Is it only the growth-rate-calc job that is reporting this?
Yes, looks like it!
Did it start reporting this almost immediately after starting growth-rate-calc job?
Did you make any changes to the config.ini, specifically the
The only change that I recall making is increasing
stirring to 50, as I had issues with the fan only working intermittently. That’s as an override in
samples_per_second is set to
shared config.ini, and there are no overrrides in
I’m sorry that I don’t have a better answer for you! If it’s not affecting the results: great. I am thinking it’s a one off problem, but I’ll make an issue around this problem to further track it. I am curious if in your next experiment, it happens again.
Hi Cam, no problem at all. If it happens again I’ll see if I can dig deeper myself to see if I can find something. Hopefully it was just a one off!