I started my first Pioreactor experiment (control testing) a couple of hours ago , after finally finding the time to prepare some YPD media and sterilizing it in a pressure cooker.
Everything seems to work fine so far, except that I’m getting a “Lost contact with the MQTT server” roughly every two minutes, even though it then almost immediately reconnects? It doesn’t seem to affect any of the readings, but is leading to a pretty noisy event log:
Hm, I’m not sure what’s causing that. Is worker1 your leader Pioreactor, too? Can you tell me the software version you are using (on the /updates page, titled Version installed)?
Is it only the growth-rate-calc job that is reporting this?
Yes, looks like it!
Did it start reporting this almost immediately after starting growth-rate-calc job?
Yes.
Did you make any changes to the config.ini, specifically the samples_per_second parameter?
The only change that I recall making is increasing initial_duty_cycle for stirring to 50, as I had issues with the fan only working intermittently. That’s as an override in config_leader.ini.
samples_per_second is set to 0.2 in shared config.ini, and there are no overrrides in config_worker1.ini.
I’m sorry that I don’t have a better answer for you! If it’s not affecting the results: great. I am thinking it’s a one off problem, but I’ll make an issue around this problem to further track it. I am curious if in your next experiment, it happens again.