Low Dilution Rate Chemostat

I am trying to run some yeast chemostats at a low dilution rate (0.10hr-1 and 0.05hr-1) and I am having issues getting growth rates matching the dilution rate. Does anyone have any idea why this might be happening or maybe some pointers?

Hm! We’ve haven’t done a comparison of dilution rate vs growth rate in a chemostat in house. In theory, they should be the same, but that makes a few assumptions including constant dilution. In the Pioreactor, we dilute via discrete drops at specific intervals. That may change the dynamics? I’m not sure myself (something-something differential equations).

I’m curious what other professionals think, too.