Here is the data for a successful calibration
{
“type”: “od_90”,
“created_at”: “2024-04-06T15:44:47.370356Z”,
“pioreactor_unit”: “P07”,
“name”: “od-cal-2024-04-06”,
“angle”: “90”,
“maximum_od600”: 3.5,
“minimum_od600”: 0.006,
“minimum_voltage”: 0.022968408136512176,
“maximum_voltage”: 3.5133738521462625,
“curve_type”: “poly”,
“curve_data_”: [
-0.07447459801429754,
1.2727848927491558,
0.014597710176138248
],
“voltages”: [
3.5133738521462625,
2.811935213414734,
2.1315097991251517,
2.091689269093525,
1.5210639433462623,
1.156651410925214,
1.142835162859364,
0.762304168393797,
0.5730418512034993,
0.5872258216525401,
0.40551942036141825,
0.3186521807097925,
0.3052613380685405,
0.2240220041962817,
0.1781993794927824,
0.1725932837868048,
0.13150771376980047,
0.10409852238535953,
0.10493012509821495,
0.08642839086756976,
0.06906339515624996,
0.06630502205993102,
0.06332040625845456,
0.04694884702612076,
0.022968408136512176
],
“od600s”: [
3.5,
2.5,
1.9444444444444444,
1.7,
1.2142857142857142,
0.9444444444444444,
1.0,
0.7142857142857143,
0.5555555555555556,
0.42,
0.3,
0.23333333333333334,
0.25,
0.17857142857142858,
0.1388888888888889,
0.14,
0.1,
0.07777777777777778,
0.07,
0.05,
0.03888888888888889,
0.035,
0.025,
0.019444444444444445,
0.006
],
“ir_led_intensity”: 50.0,
“pd_channel”: “2”
}
Yes I did and all the Pios including the ones with the issue of this topic passed all the tests.
I assume by sayin “ratio” to refer to the number of OD values registered. If that is the case, yes for a 10ml start, 4ml dilution, there is 2-to-1 inferred to manual OD ratio(i.e., I registered 1 “manual” OD value after 2 “inferred” OD values). Let me know if I understood it correctly.
EDIT: I would like to state that, I do not have any data for the “faulty” HATs as I had to stop calibration before saving the outcomes, Hence, I only have the picture I provided at the beginning.