Hi @sharknaro
What do you mean by “changing the HAT unit”? Like physically swapping the HAT for another HAT? I haven’t seen anything like this before. It might be a software bug instead.
You have a lot of manual samples in your data, which is fine, but there could be an edge case here. Can you tell us what combination of parameters you are using at the start of the OD calibration?
Anything else you can share about the process? Are you diluting the samples for the manual OD600?
We recognize our OD calibration needs some attention, so we’d be happy to hear feedback about the algorithm (and you’ve already provided some feedback in Advice for OD calibration range).