Hello, we’re going to grow and express GFP with IPTG inside BL-21 strain of E.coli today, we are wondering if anyone has tried to attempt this? What were your results? Was is done in the 40ml setup or the 20ml?
Otherwise, has anyone tested the cell health of of DH5-Alpha or BL-21?
And also, how does the OD of the Pioreactor relate to the from a typical spectrometer, because we are trying to figure out the optimal OD at which we can add IPTG.