Hi @MPeruzzo, welcome!
Is it correct that you are using the code here? Absolute OD targets for dosing automation - #2 by CamDavidsonPilon
Let me try to debug it and I’ll post an update in that thread (I’ll ping you, too).
Yea, sounds like it another OD calibration should be done. If you think there is a problem, I encourage you to open a new forum post here where we can discuss it!