What does anyone think would be the best way to increase the maximum OD readable (for e coli)? Increasing the intensity of the LED? Would there be any applicability for the adafruit spectrometer? Just looking to run a high density culture and would like to get the maximum OD as high as possible.
Thanks.
Worth noting that this is possible. I was just chatting to a Hamilton rep that pointed me to their total cell density sensor that can read up to 300 g/L cell density: https://www.hamiltoncompany.com/knowledge-base/article/dencytee-arc-total-cell-density-measurement-principle. Might be good to try the different wavelength like they use? Don’t know what one the pioreactor currently uses
The Pioreactor uses 900nm, and specifically side-scatter (90deg). The tool linked uses back-scatter for super dense cultures (~0deg). One could redesign the top-vial-holder to use the second PD (currently in the REF pocket) to measure near ~0deg signal.
The V1.0 top-vial-holder had a 45deg scatter for dense cultures, but we phased this out.